An in vitro comparison of fracture resistance of immature teeth subjected to apexification using three different bioactive materials

To compare the fracture resistance of immature teeth subjected to apexification with three different bioactive materials. Sixty non-carious, single-rooted premolar straight canals were decoronated at cementoenamel junction, standardizing 13 mm length. After access preparation, accessed #10 K-file followed by biomechanical preparation using Ni-Ti ProTaper Gold rotary files upto F3 and irrigation was done 2 mL 3% NaOCl, 17% EDTA 5 ml saline. simulate roots open apices, #1–5 Peeso reamers passed through apex enlarging 1.5 diameter. Later, samples randomly divided into 4 groups 5mm apical plug in each group, Group I: GuttaFlow Bioseal, II: Biodentine, III: EndoSeal MTA IV: Control group. Samples filled an 5mm. remaining part root obturated gutta-percha cones AH Plus sealer lateral condensation. stimulate periodontal ligament lining surfaces, C-Silicone light body impression material used. The stored for 1 week 100% humidity 37˚C ensure that set environment clinical situation. evaluated universal testing machine. II (Biodentine) showed highest reisistance I (GuttaFlow BioSeal) III (EndoSeal MTA) Biodentine values Bioseal least shown MTA.